PIP2;3, aquaporin, plasma membrane intrinistic protein 2-3

• Catalog number
  AS09 506
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Inquire
• Immunogen
  KLH-conjugated peptide derived from Oryza sativa PIP2;3 Q7XUA6
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
• Verified applications
  ELISA (ELISA), western blot (WB), immunogold (IG)
• Connected products
  collection of antibodies to plasma membrane proteins AS09 505 | anti-PIP1s | aquaporinAS09 507 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1AS09 508 | anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5Plant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 8000 (ELISA), 1: 2000 with standard ECL (WB), 1: 100 (IG)
• Molecular weight expected аpparent
  31.9 | 26-29 kDa
• Verified reactivity
  Oryza sativa, Zea mays
• Possible reactivity
  monocots
• No reactivity
  Arabidopsis thaliana
• Supplementary information
  Antibody works very well on samples from roots. Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
• References
  Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.
• Scientific context
  PIP2;3 is an aquaporin which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: plasma membrane intrinsic protein 2-3, OsPIP2;3
• Notes
  0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.For immunogold technique, secondary antibodies conjugated with coloidal gold, 10 nm particle size were used.Protocol for isolation of plasma membrane can be found here.
• Protein number
  Q6Z2T3
• TAIR number
  Refer to NCBI
• Hyperlink
• Description
  Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 

• Gene target
  PIP2   aquaporin   plasma   membrane   intrinistic   protein   2-3  
• Gene symbol
  TRC-GCA2-3, TRW-CCA3-2, TRG-TCC2-3, TRP-TGG3-3, TRR-ACG2-3, TRI-TAT2-3, TRM-CAT3-2, TRQ-CTG3-2, TRK-TTT3-2, TRD-GTC2-3
• Short name
  PIP2 3, aquaporin, plasma intrinistic protein 2-3
• Alternative name
  PIP2;3, aquaporin, plasma membrane intrinistic protein 2-3

Gene info

• Identity
  HGNC:34666
• Gene
  TRC-GCA2-3
• Long gene name
  tRNA-Cys (anticodon GCA) 2-3
• Synonyms gene
  • TRNAC9
  • TRC-GCA2-2
• Synonyms gene name
  • transfer RNA cysteine 9 (anticodon GCA)
  • transfer RNA-Cys (GCA) 2-2
  • transfer RNA-Cys (GCA) 2-3
• Synonyms
  • tRNA-Cys-GCA-2-3
• GenBank acession
  • HG983687
• Locus
  17q12
• Discovery year
  2008-08-29
• Entrez gene record
  100189105
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34607
• Gene
  TRW-CCA3-2
• Long gene name
  tRNA-Trp (anticodon CCA) 3-2
• Synonyms gene
  • TRNAW2
  • TRW-CCA3-3
• Synonyms gene name
  • transfer RNA tryptophan 2 (anticodon CCA)
  • transfer RNA-Trp (CCA) 3-3
  • transfer RNA-Trp (CCA) 3-2
• Synonyms
  • tRNA-Trp-CCA-3-2
• GenBank acession
  • HG984068
• Locus
  6p22.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189048
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34703
• Gene
  TRG-TCC2-3
• Long gene name
  tRNA-Gly (anticodon TCC) 2-3
• Synonyms gene
  • TRNAG13
• Synonyms gene name
  • transfer RNA glycine 13 (anticodon UCC)
  • transfer RNA-Gly (TCC) 2-3
• Synonyms
  • tRNA-Gly-TCC-2-3
• GenBank acession
  • HG983824
• Locus
  1q23.3
• Discovery year
  2008-08-29
• Entrez gene record
  100189141
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34903
• Gene
  TRP-TGG3-3
• Long gene name
  tRNA-Pro (anticodon TGG) 3-3
• Synonyms gene
  • TRNAP19
  • TRP-TGG3-2
• Synonyms gene name
  • transfer RNA proline 19 (anticodon UGG)
  • transfer RNA-Pro (TGG) 3-2
  • transfer RNA-Pro (TGG) 3-3
• Synonyms
  • tRNA-Pro-TGG-3-3
• GenBank acession
  • HG983998
• Locus
  16p13.3
• Discovery year
  2008-08-29
• Entrez gene record
  100189335
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34660
• Gene
  TRR-ACG2-3
• Long gene name
  tRNA-Arg (anticodon ACG) 2-3
• Synonyms gene
  • TRNAR10
• Synonyms gene name
  • transfer RNA arginine 10 (anticodon ACG)
  • transfer RNA-Arg (ACG) 2-3
• Synonyms
  • tRNA-Arg-ACG-2-3
• GenBank acession
  • HG983599
• Locus
  6p22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189099
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34693
• Gene
  TRI-TAT2-3
• Long gene name
  tRNA-Ile (anticodon TAT) 2-3
• Synonyms gene
  • TRNAI6
• Synonyms gene name
  • transfer RNA isoleucine 6 (anticodon UAU)
  • transfer RNA-Ile (TAT) 2-3
• Synonyms
  • tRNA-Ile-TAT-2-3
• GenBank acession
  • HG983866
• Locus
  6p22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189131
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34673
• Gene
  TRM-CAT3-2
• Long gene name
  tRNA-Met (anticodon CAT) 3-2
• Synonyms gene
  • TRNAM8
• Synonyms gene name
  • transfer RNA methionine 8 (anticodon CAU)
  • transfer RNA-Met (CAT) 3-2
• Synonyms
  • tRNA-Met-CAT-3-2
• GenBank acession
  • HG983953
• Locus
  6p22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189111
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34702
• Gene
  TRQ-CTG3-2
• Long gene name
  tRNA-Gln (anticodon CTG) 3-2
• Synonyms gene
  • TRNAQ13
• Synonyms gene name
  • transfer RNA glutamine 13 (anticodon CUG)
  • transfer RNA-Gln (CTG) 3-2
• Synonyms
  • tRNA-Gln-CTG-3-2
• GenBank acession
  • HG983723
• Locus
  1q21.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189140
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34994
• Gene
  TRK-TTT3-2
• Long gene name
  tRNA-Lys (anticodon TTT) 3-2
• Synonyms gene
  • TRNAK34
• Synonyms gene name
  • transfer RNA lysine 34 (anticodon UUU)
  • transfer RNA-Lys (TTT) 3-2
• Synonyms
  • tRNA-Lys-TTT-3-2
• GenBank acession
  • HG983932
• Locus
  1q32.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189425
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34856
• Gene
  TRD-GTC2-3
• Long gene name
  tRNA-Asp (anticodon GTC) 2-3
• Synonyms gene
  • TRNAD13
• Synonyms gene name
  • transfer RNA aspartic acid 13 (anticodon GUC)
  • transfer RNA-Asp (GTC) 2-3
• Synonyms
  • tRNA-Asp-GTC-2-3
• GenBank acession
  • HG983667
• Locus
  1q23.3
• Discovery year
  2008-08-29
• Entrez gene record
  100189290
• Classification
  • Cytoplasmic transfer RNAs

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