TIP1;1, TIP1;2, tonoplast intrinistic protein 1-1, 1-2 (gamma)

• Catalog number
  AS09 493
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Inquire
• Immunogen
  KLH-conjugated synthetic peptide conserved in Raphanus sativus TIP1;1 and TIP1;2 (protein accesion number available in Suga et al. 2001). Peptide is also conserved in Arabidopsis thaliana TIP1-1 P25818, At2g36830, TIP1-2 Q41963, At3g26520
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
• Verified applications
  ELISA (ELISA), western blot (WB)
• Connected products
  collection of antibodies to tonoplast proteins AS09 492 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)AS09 494 | anti-TIP2;1 (delta) | Delta-VM23AS09 510 | anti-TIP2;1 | tonoplast intrinistic protein 2-1AS09 511 | anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal)AS09 493P | TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma) | blocking peptidePlant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
• Molecular weight expected аpparent
  25.8| 23 kDa
• Verified reactivity
  Arabidopsis thaliana, Raphanus sativus
• Possible reactivity
  dicots including: Brassica napus, Gossypium hirsutum, Ricinus communis, monocots including: Hordeum vulgare, Oryza sativa, trees: Populus trichocarpa,
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
• References
  Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244. Suga et al. (2001). Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporings in radish organs. Planta 212:294-304.
• Scientific context
  TIPs belong to MIP/aquaporin protein family.Alternative names: Aquaporin TIP1-1,gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP, gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP, tonoplast intrinsic protein, root-specific RB7, gamma-tonoplast intrinsic protein 2, gamma-TIP2, salt stress-induced tonoplast intrinsic protein
• Notes
  0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for isolation of vacuolar membranes can be found here.
• Protein number
  P25818 , Q41963
• TAIR number
  AT2G36830
• Hyperlink

• Gene target
  TIP1   TIP1   tonoplast   intrinistic   protein   1-1   1-2   gamma  
• Gene symbol
  IGHVIII-2-1, TRH-GTG2-1, TRUND-NNN9-1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN2-1, TRUND-NNN7-1, SLC2A4RG, SLC9A3R2
• Short name
  TIP1 1, TIP1 2, tonoplast intrinistic protein 1-1, 1-2 (gamma)
• Alternative name
  TIP1;1, TIP1;2, tonoplast intrinistic protein 1-1, 1-2 (g)

Gene info

• Identity
  HGNC:5695
• Gene
  IGHVIII-2-1
• Long gene name
  immunoglobulin heavy variable (III)-2-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-2-1
  • immunoglobulin heavy variable (III)-2-1 pseudogene
• Synonyms
  • IGHV(III)-2-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28355
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152321

Gene info

• Identity
  HGNC:34918
• Gene
  TRH-GTG2-1
• Long gene name
  tRNA-His (GTG) 2-1
• Synonyms gene
  • TRNAH11
• Synonyms gene name
  • transfer RNA histidine 11 (anticodon GUG)
  • transfer RNA-His (GTG) 2-1
• Synonyms
  • tRNA-His-GTG-2-1
• GenBank acession
  • HG983841
• Locus
  1
• Discovery year
  2008-08-29
• Entrez gene record
  100189350
• Classification
  • Low confidence cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:50821
• Gene
  TRUND-NNN9-1
• Long gene name
  tRNA-undetermined (NNN) 9-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 9-1
• GenBank acession
  • HG984098
• Locus
  2
• Discovery year
  2014-06-20
• Entrez gene record
  103482526
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50814
• Gene
  TRUND-NNN2-1
• Long gene name
  tRNA-undetermined (NNN) 2-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 2-1
• Synonyms
  • tRNA-Und-NNN-2-1
• GenBank acession
  • HG984091
• Locus
  8q22.2
• Discovery year
  2014-06-20
• Entrez gene record
  103482523
• Pubmed identfication
  • 18984615
• Classification
  • Low confidence cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:15930
• Gene
  SLC2A4RG
• Long gene name
  SLC2A4 regulator
• Synonyms
  • GEF
  • HDBP1
  • Si-1-2
  • Si-1-2-19
• Synonyms name
  • GLUT4 enhancer factor
  • Huntington's disease gene regulatory region-binding protein 1
• GenBank acession
  • AF249267
• Locus
  20q13.33
• Discovery year
  2001-06-21
• Entrez gene record
  56731
• Pubmed identfication
  • 10825161
• RefSeq identity
  • NM_020062
• VEGA ID
  OTTHUMG00000032997

Gene info

• Identity
  HGNC:11076
• Gene
  SLC9A3R2
• Long gene name
  SLC9A3 regulator 2
• Synonyms gene name
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 2
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
  • solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 2
• Synonyms
  • SIP-1
  • TKA-1
  • NHERF-2
  • E3KARP
• GenBank acession
  • AF004900
• Locus
  16p13.3
• Discovery year
  1999-02-26
• Entrez gene record
  9351
• Pubmed identfication
  • 9054412
  • 9671706
• Classification
  • PDZ domain containing
• VEGA ID
  OTTHUMG00000176956

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