PIP2;1, PIP2;2, PIP2;3, plasma membrane intrinistic protein 2-1,2-2,2-3

• Catalog number
  AS09 491
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Inquire
• Immunogen
  KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIP2 proteins: AtPIP2-1, At3g53420 , AtPIP2-2 P43287, At2g37170 , AtPIP2-3 P30302, At2g37180
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
• Verified applications
  ELISA (ELISA), western blot (WB)
• Connected products
  collection of antibodies to plasma membrane proteins AS09 488 | anti-PIP2;1 | aquaporin PIP2;1AS09 489 | anti-PIP1;1, PIP1;2, PIP1;3 | aquaporinsAS09 490 | anti-PIP2;2 | plasma membrane aquaporin 2bPlant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
• Molecular weight expected аpparent
  30.4 | 28 (PIP2-1,PIP2-2,PIP2-3) kDa
• Verified reactivity
  Arabidopsis thaliana, Gromphadorhina coquereliana, Raphanus sativus
• Possible reactivity
  Camelina sativa, Brassica napus, Cucumis sativus, Glycine hispida, Gossypium hirsutum, Hedychium coronarium, Mimosa saman, Nicotiana glauca, Petunia hybrida, Pisum sativum, Ricinus communis, trees: Populus tremula x Populus tremloides, moss: Physcomitrella patens
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
• References
  Chowanski et al. (2015). Cold induced changes in lipid, protein and carbohydrate levels in the tropical insect Gromphadorhina coquereliana. Comp Biochem Physiol A Mol Integr Physiol. 2015 May;183:57-63. doi: 10.1016/j.cbpa.2015.01.007. Epub 2015 Jan 23.
• Scientific context
  PIP2;2 is a plasma membrane aquaporin. Alternative names of isoforms: aquaporin PIP2-1, plasma membrane intrinsic protein 2a, PIP2a, aquaporin PIP2-2, plasma membrane intrinsic protein 2b, PIP2b, TMP2b, Aquaporin PIP2-3, plasma membrane intrinsic protein 2c, PIP2c, TMP2C, RD28-PIP, water stress-induced tonoplast intrinsic protein, (WSII-TIP)
• Notes
  0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for plasma membrane isolation can be found here.
• Protein number
  P43286 , P43287 , P30302
• TAIR number
  AT3G53420
• Hyperlink
• Description
  Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 

• Gene target
  PIP2   PIP2   PIP2   plasma   membrane   intrinistic   protein   2-1   2-2   2-3  
• Gene symbol
  MIR1-2, MIR7-2, MIR329-2, MIR512-2, MIR521-2, MIR1289-2, MIR509-2, IGHVIII-2-1, KRTAP13-2, RNU6-2
• Short name
  PIP2 1, PIP2 2, PIP2 3, plasma intrinistic protein 2-1 2-2 2-3
• Alternative name
  PIP2;1, PIP2;2, PIP2;3, plasma membrane intrinistic protein 2-1,2-2,2-3

Gene info

• Identity
  HGNC:31500
• Gene
  MIR1-2
• Long gene name
  microRNA 1-2
• Synonyms gene
  • MIRN1-2
• Synonyms
  • hsa-mir-1-2
• Locus
  18q11.2
• Discovery year
  2004-04-23
• Entrez gene record
  406905
• RefSeq identity
  • NR_029662
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:31639
• Gene
  MIR7-2
• Long gene name
  microRNA 7-2
• Synonyms gene
  • MIRN7-2
• Synonyms
  • hsa-mir-7-2
• Locus
  15q26.1
• Discovery year
  2004-04-23
• Entrez gene record
  407044
• RefSeq identity
  • NR_029606
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:32051
• Gene
  MIR329-2
• Long gene name
  microRNA 329-2
• Synonyms gene
  • MIRN329-2
• Synonyms
  • hsa-mir-329-2
• Locus
  14q32.31
• Discovery year
  2005-07-11
• Entrez gene record
  574409
• RefSeq identity
  • NR_029968
• Classification
  • MicroRNA MIR329 family

Gene info

• Identity
  HGNC:32091
• Gene
  MIR512-2
• Long gene name
  microRNA 512-2
• Synonyms gene
  • MIRN512-2
• Synonyms
  • hsa-mir-512-2
• Locus
  19q13.42
• Discovery year
  2005-07-15
• Entrez gene record
  574459
• RefSeq identity
  • NR_030181
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:32113
• Gene
  MIR521-2
• Long gene name
  microRNA 521-2
• Synonyms gene
  • MIRN521-2
• Synonyms
  • hsa-mir-521-2
• Locus
  19q13.42
• Discovery year
  2005-07-15
• Entrez gene record
  574481
• RefSeq identity
  • NR_030203
• Classification
  • MicroRNA MIR515 family

Gene info

• Identity
  HGNC:35282
• Gene
  MIR1289-2
• Long gene name
  microRNA 1289-2
• Synonyms gene
  • MIRN1289-2
• Synonyms
  • hsa-mir-1289-2
• Locus
  5q31.1
• Discovery year
  2008-11-03
• Entrez gene record
  100302134
• RefSeq identity
  • NR_031621
• Classification
  • MicroRNA MIR1289 family

Gene info

• Identity
  HGNC:33641
• Gene
  MIR509-2
• Long gene name
  microRNA 509-2
• Synonyms gene
  • MIRN509-2
• Synonyms
  • hsa-mir-509-2
• Locus
  Xq27.3
• Discovery year
  2007-10-24
• Entrez gene record
  100126301
• RefSeq identity
  • NR_030586
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:5695
• Gene
  IGHVIII-2-1
• Long gene name
  immunoglobulin heavy variable (III)-2-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-2-1
  • immunoglobulin heavy variable (III)-2-1 pseudogene
• Synonyms
  • IGHV(III)-2-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28355
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152321

Gene info

• Identity
  HGNC:18923
• Gene
  KRTAP13-2
• Long gene name
  keratin associated protein 13-2
• Synonyms
  • KAP13-2
• GenBank acession
  • AP001708
• Locus
  21q22.11
• Discovery year
  2003-02-21
• Entrez gene record
  337959
• Pubmed identfication
  • 12359730
• Classification
  • Keratin associated proteins
• VEGA ID
  OTTHUMG00000057793

Gene info

• Identity
  HGNC:34270
• Gene
  RNU6-2
• Long gene name
  RNA, U6 small nuclear 2
• Synonyms
  • U6-2
• Locus
  19p13.3
• Discovery year
  2008-06-10
• Entrez gene record
  103625684
• Pubmed identfication
  • 12711679
• RefSeq identity
  • NR_125730
• Classification
  • Small nuclear RNAs

Similar products