Human Ghrelin (GHRL) ELISA Kit, 96 well plate
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Catalog numberEKU04361
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Price:
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Size10x96T
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Verified reactivityHomo sapiens (Human)
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Detection limits123.5-10000 pg/mL
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Sensitivity limit49,5 pg/mL
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Samples to be analyzedSerum, plasma, tissue homogenates, cell lysates, saliva, cell culture supernates and other biological fluids
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Assay duration2 hours
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Verified applicationsELISA
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Assay classCompetitive
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ELISA detectionColorimetric
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Protein numberQ9UBU3
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Alternate protein numberPlease refer to SwissProt
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Gene number51738
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Gene nameGhrelin
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Alternate gene nameMTLRP; Growth Hormone-Releasing Peptide
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Estimated production time7-11 business days
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Shipping requirementsBlue ice
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Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for8 months
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ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Ghrelin (GHRL). No significant cross-reactivity or interference between Ghrelin (GHRL) and analogues was observed.
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ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ghrelin (GHRL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ghrelin (GHRL) and unlabeled Ghrelin (GHRL) (Standards or samples) with the pre-coated antibody specific to Ghrelin (GHRL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ghrelin (GHRL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ghrelin (GHRL) in the sample.
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ProtocolPlease see ELISA's datasheet, otherwise contact us
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Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ghrelin (GHRL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main areaSignal transduction;Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism;
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WarningsThe Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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NotesFor research use only. Not for diagnostic procedures.
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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DescriptionGhrelin, GHRL gene, obestatin prepropeptide or lenomorelin from ghrelinergic cells in the gastrointestinal tract. GHRL is a neuropeptide in the central nervous system regulating appetite. Ghrelin is regulating the distribution and rate of use of body energy. A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
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Gene target
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Gene symbolGHRL, GHRLOS, LINC00852
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Short nameGhrelin (GHRL) ELISA Kit, 96
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TechniqueELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Hostantigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesHuman, Humans
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Alternative nameH. sapiens Ghrelin (ghrelin/obestatin prepropeptide) Enzyme-linked immunosorbent assay reagent, 96 well plate
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Alternative techniqueelisas, kits
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Alternative to gene targetghrelin/obestatin prepropeptide, GHRL and IDBG-18011 and ENSG00000157017 and 51738, ghrelin receptor binding, Extracellular, Ghrl and IDBG-178411 and ENSMUSG00000064177 and 58991, GHRL and IDBG-646311 and ENSBTAG00000012328 and 281192
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Gene info
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Identity
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Gene
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Long gene nameghrelin and obestatin prepropeptide
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Synonyms gene name
- ghrelin, growth hormone secretagogue receptor ligand
- ghrelin/obestatin prepropeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-01-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Neuropeptides
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameghrelin opposite strand/antisense RNA
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Synonyms gene name
- ghrelin opposite strand RNA (non-protein coding)
- ghrelin opposite strand/antisense RNA (non-protein coding)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2007-11-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long non-coding RNAs with non-systematic symbols
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelong intergenic non-protein coding RNA 852
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Synonyms gene
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Synonyms gene name
- chromosome 3 open reading frame 42
- ghrelin opposite strand/antisense RNA 2 (tail to tail)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-01-10
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Entrez gene record
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Pubmed identfication
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Classification
- Long intergenic non-protein coding RNAs
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VEGA ID
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data