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Verified reactivity
Chicken (Gallus)
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Detection limits
39.06-10000 pg/mL
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Sensitivity limit
17,31 pg/mL
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Samples to be analyzed
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
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Assay duration
2 hours
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Verified applications
CLIA
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Assay class
Competitive
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ELISA detection
Chemiluminescent
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Protein number
Please refer to Uniprot
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Alternate protein number
Please refer to SwissProt
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Gene number
Please refer to GenBank
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Gene name
Ghrelin
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Alternate gene name
MTLRP; Growth Hormone-Releasing Peptide
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Estimated production time
7-11 business days
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Shipping requirements
Blue ice
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Storage recommendation
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for
8 months
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ELISA s specificity
This assay has high sensitivity and excellent specificity for detection of Ghrelin (GHRL). No significant cross-reactivity or interference between Ghrelin (GHRL) and analogues was observed.
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ELISA s cross reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principle
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Ghrelin (GHRL). A competitive inhibition reaction is launched between biotin labeled Ghrelin (GHRL) and unlabeled Ghrelin (GHRL) (Standards or samples) with the pre-coated antibody specific to Ghrelin (GHRL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ghrelin (GHRL) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Ghrelin (GHRL) level in the sample or standard.
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Protocol
Please see ELISA's datasheet, otherwise contact us
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Precision of the test
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ghrelin (GHRL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QC
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main area
Signal transduction;Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism;
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Warnings
Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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Notes
For research use only. Not for diagnostic procedures.
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Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
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Description
Ghrelin, GHRL gene, obestatin prepropeptide or lenomorelin from ghrelinergic cells in the gastrointestinal tract. GHRL is a neuropeptide in the central nervous system regulating appetite. Ghrelin is regulating the distribution and rate of use of body energy. A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals.
A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.