Goat Biotin antibody (HRP)
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Catalog number
60R-1003
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Price
Please ask
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Size
1 mg
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Applications
User optimized
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Product Type
Primary Antibodies
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Product Subtype
Conjugated Polyclonal Antibodies
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Research Area
Miscellaneous
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Immunogen
Biotin
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Specificity
NA
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Cross Reactivity
NA
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Clone
NA
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Concentration
1.0 mg/ml
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Form Buffer
Lyophilized powder containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.1% (v/v) Kathon CG. Protease/IgG free, pH 7.2. Rehydrate with 1.1 ml of deionized water. Prepare fresh working dilution daily.
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Storage
Store lyophilized material at 2-8 deg C.
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Shipping Info
Blue Ice
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
Biotinylated, HRP Conjugated
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Latin name
Capra aegagrus hircus
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French translation
anticorps
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Short name
Goat antibody ( )
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Technique
Antibody, Goat, antibodies against human proteins, antibodies for, goats
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
NA
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Label
Biotin
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Species
Goats
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Alternative name
Biotin antibody (HRP)
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Alternative technique
antibodies, caprine
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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