Biotin reagent HRP

• Catalog number
  65C-CE0202
• Price
  Please ask
• Size
  5 mg

• Category
  Proteins
• Antibody Subtype
  Conjugated Proteins
• Area of research
  Miscellaneous
• Reactivity
  N/A
• Conjugate Tag
  HRP
• Type of protein
  Synthetic
• Method of Purification
  Biotin reagent (HRP) was purified by ion exchange chromatography followed by dialysis.
• Form Buffer
  Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamycin sulfate. DO NOT add NaN3. I mMunoglobulin and protease free.
• Storage
  Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  ELISA; IHC; WB
• Usage Recommendations
  ELISA: 1:20,000-1:200,000, IHC: 1:1,000-1:5,000, WB: 1:10,000-1:40,000
• URL
• More info
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
• Conjugation
  Biotinylated, HRP Conjugated

• Gene target
  reagent  
• Short name
  reagent
• Label
  Biotin
• Alternative name
  HRP conjugated biotin labelling reagent

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