Human K-ras Gene Mutations Detection Kit (Multiplex Fluorescence PCR)

• Catalog number
  SpaceGen-006
• Price
  Please ask
• Size
  1 Kit

• Description
  Human K-ras Gene Mutations Detection Kit (Multiplex Fluorescence PCR) is employed to detect K-ras mutation that results from excessive growth signals of p21-ras proteins and causes variety of cancers,
• Other product
• Specifications
  10tests/kit
• Other Name
  Detection Kit for Human KRAS Gene Mutation
• Detection Process
  Multiplex Fluorescence PCR
• Samples Types
  Paraffin-embedded tissue, fresh tumor tissue, fresh frozen tumors, blood, pleural effusion, etc.
• Gene
  KRAS
• Reactivity
  Human
• Advantages and Characteristics
  Easy operation: This kit only takes one step to add the sample, which can avoid pollution and save time, and it takes only 90 minutes to complete the detection, High sensitivity: This kit can effectively detect the common 7 kinds of mutational sites; Strong Specificity : This kit are able to tolerate DNA in the background of 100ng wild-type genome, without nonspecific amplification., Comprehensive Inspection: This kit can accurately detect DNA samples with a mutation content of 0.1% in the background of 10ng genome.
• Use
  CE-IVD
• Additional description
  The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol. cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
• Test
  Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
• Group
  PCR, polymerase chain reaction
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.

• Gene target
  K-ras   Gene   Mutations   Kit  
• Short name
  K-ras Mutations Kit (Multiplex PCR)
• Technique
  detection, multiplex, PCR, multiplexes, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
• Species
  Human, Humans
• Alternative name
  K-ras Gene Mutations Detection Kit
• Alternative technique
  pcrkits, kits, dna-amplification
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Disease
  variety of cancers,

MeSH Data

• Name
  Polymerase Chain Reaction
• Concept
  Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
• Tree numbers
  • E05.393.620.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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