Real-time PCR multiplex detection kit for Dengue virus, Zika virus and Chikungunya virus
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Other size
please contact us to order other different size
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Description
The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol. Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Short name
Real-time PCR multiplex kit for Dengue virus, Zika virus Chikungunya virus
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Technique
detection, multiplex, Real-time, PCR, multiplexes, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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Species
Virus, Viruses
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Alternative name
Real-time PCR test kit multiplex quantification reagent to measure Dengue virus, ZIKV virus and Chikungunya virus
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Alternative technique
pcrkits, rtpcr, kits, dna-amplification
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Virus
chikungunya, dengue, zika
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MeSH Data
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Name
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Concept
Scope note:
Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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