Human EGFR Gene Mutations Detection Kit (Multiplex Fluorescence PCR)

  • Catalog number
    SpaceGen-005
  • Price
    Please ask
  • Size
    1 Kit
  • Description
    Human EGFR Gene Mutations Detection Kit (Multiplex Fluorescence PCR) is used to detect the EGFR mutation in a patient in ordre to determine if a patient can apply EGFR-TKI therapy, EGFR mutation is responsable for cancer cells proliferation and relevant signal transduction, erlotinib, icotinib and other EGFR-TKI and anti-EGFR antibodies have been developped for cancer treatment.
  • Specifications
    10tests/kit
  • Other Name
    Detection Kit for Human EGFR Gene Mutation
  • Detection Process
    Multiplex Fluorescence PCR
  • Samples Types
    Paraffin-embedded tissue, fresh tumor tissue, fresh frozen tumors, blood, pleural effusion, etc.
  • Gene
    EGFR, EGFR-TKI
  • Reactivity
    Human
  • Advantages and Characteristics
    Easy operation: this kit only takes one step to add the sample, which can avoid pollution and save time, and it takes only 90 minutes to complete the detectionComprehensive Inspection: This kit can effectively detect the common 29 kinds of mutational sites, Strong Specificity: This kit are able to tolerate DNA in the background of 100ng wild-type genome, without nonspecific amplification, High snsitivity: This kit can accurately detect DNA samples with a mutation content of 0.1% in the background of 10ng genome.
  • Use
    CE-IVD
  • Additional description
    The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol. cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
  • Test
    Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
  • Group
    PCR, polymerase chain reaction
  • About
    TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
  • Gene target
    EGFR   Gene   Mutations   Kit  
  • Gene symbol
    EGFR-AS1, EGILA, EGFR, RHBDF1
  • Short name
    EGFR Mutations Kit (Multiplex PCR)
  • Technique
    detection, multiplex, PCR, multiplexes, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
  • Species
    Human, Humans
  • Alternative name
    Gene Mutations Detection Kit
  • Alternative technique
    pcrkits, kits, dna-amplification
  • Alternative to gene target
    epidermal growth factor receptor, ERBB and ERBB1 and HER1 and mENA and PIG61, EGFR and IDBG-16933 and ENSG00000146648 and 1956, transferase activity, nuclei, Egfr and IDBG-151194 and ENSMUSG00000020122 and 13649, EGFR and IDBG-644035 and ENSBTAG00000011628 and 407217
  • Disease
    Lung Cancer, varietyof cancer
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    EGFR interacting lncRNA
  • Synonyms
  • Locus
  • Discovery year
    2019-09-27
  • Pubmed identfication
  • Classification
    • Long non-coding RNAs with non-systematic symbols
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Methods for using more than one primer set in a polymerase chain reaction to amplify more than one segment of the target DNA sequence in a single reaction.
  • Tree numbers
    • E05.393.620.500.487
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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