Glutathione Fluorescent Detection Kit

Glutathione Fluorescent Detection Kit

• Description: ICT’s Glutathione Fluorescent Detection Kit is designed to quantitatively measure reduced state glutathione (GSH), and oxidized state glutathione (GSSG) present in a variety of samples. Sample types validated include: human whole blood; serum; EDTA and heparin plasma; urine; and isolated erythrocytes. Most cell lysates and tissue homogenates should also be compatible.
• Assay Principle: 1. Prepare samples; treat with SSA to remove thiol-bearing protein., 2. Prepare standard curve and blanks., 3. If measuring oxidized GSH (GSSG), treat blanks, samples, and standards with 2VP., 4. Add 50 µL blanks, standards, and samples to plate., 5. Add 25 µL Colorimetric GSH Detection Substrate., 6. Add 25 µL Reaction Mixture., 7. Mix., 8. Incubate 20 minutes at room temperature., 9. Read at 405 nm.
• Shipping Conditions: Blue Ice
• Storage Conditions: 4°C