TMB 1-Component HRP Membrane Substrate (SUBM)

TMB 1-Component HRP Membrane Substrate (SUBM)

• Description: A ready-to-use formulation containing TMB in a mildly acidic buffer. This TMB substrate is useful for membrane-based immunoblotting applications using HRP-conjugates for detection. Yields an insoluble, permanent dark blue reaction product.
• Target Antigen: Caspases, GC-rich DNA
• Assay Principle: 1. Allow product to equilibrate to room temperature (25°C) prior to use., 2. Completely cover the membrane surface with the substrate. The substrate will react with phosphatase creating a purple-blue reaction product., 3. To stop the reaction, rinse the developed membrane with distilled water., 4. For best results, the reaction should be monitored and read before the background staining becomes too intense to distinguish from the positive staining.
• pH: pH: 5.25-5.65
• Shipping Conditions: Blue Ice
• Storage Conditions: 4°C
• Shelf Life: 36 months from date of manufacture