pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets (SUBP)

• Description:

  pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets is a ready-to-use alkaline phosphatase substrate formulation to detect alkaline phosphatase-conjugated molecules in ELISA and microwell assays.

• Target Antigen:

  Apoptosis

• Assay Principle:

  1. Allow product to equilibrate to room temperature (25°C) prior to use., 2. Completely cover the membrane surface with the substrate. The substrate will react with peroxidase creating an insoluble permanent dark blue reaction product., 3. To stop the reaction, rinse the developed membrane with high quality water., 4. For best results, the reaction should be monitored and read before the background staining becomes too intense to distinguish from the positive staining.

• pH:

  pH: 9.6-10.2

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  4°C

• Shelf Life:

  36 months from date of manufacture