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TMB 1-Component HRP Membrane Substrate (SUBM)

CAT: 0013-GTR19286729Size: 100 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR19286729Size:100 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
A ready-to-use formulation containing TMB in a mildly acidic buffer. This TMB substrate is useful for membrane-based immunoblotting applications using HRP-conjugates for detection. Yields an insoluble, permanent dark blue reaction product.
Field of Research
Cancer Biology; Cell Biology
Concentration
1 X
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
1. Allow product to equilibrate to room temperature (25°C) prior to use. 2. Completely cover the membrane surface with the substrate. The substrate will react with phosphatase creating a purple-blue reaction product. 3. To stop the reaction, rinse the developed membrane with distilled water. 4. For best results, the reaction should be monitored and read before the background staining becomes too intense to distinguish from the positive staining.

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