Anti-Spectrin beta III (SPTBN2) (SPTBN2/1778)

• Description:

  Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin α and spectrinβ are present in erythrocytes, whereas spectrin α II (also designated fodrin α) and spectrinβ I (also designated fodrinβ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrinβ III is highly homologous to both spectrinβ I and spectrinβ II. Spectrinβ III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Beta III spectrin; SCA5; Spectrin beta chain brain 2; Spectrin beta non-erythrocytic 2; Spectrin non-erythroid beta chain 2; Spinocerebellar ataxia 5; SPTBN2

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  SPTBN2

• Gene ID:

  6712

• NCBI Gene ID:

  26915

• UniProt:

  O15020

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment (around aa356-475) of human SPTBN2 protein (exact sequence is proprietary)

• Target Antigen:

  Spectrin Beta-III

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  SPTBN2/1778

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  IF (verified) | IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  IF, IHC, FFPE, WB

• Field of Research:

  Cytoskeleton

• Positive Control:

  PANC-1, HeLa, PC-3 cells. Brain, Pancreas or Liver.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  246 kDa

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4