Anti-Spectrin beta III (SPTBN2) (SPTBN2/1247)

• Description:

  Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin alpha and spectrin beta are present in erythrocytes, whereas spectrin alpha II (also designated fodrin alpha) and spectrin beta I (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin beta III is highly homologous to both spectrin beta I and spectrin beta II. Spectrin beta III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Beta III spectrin; SCA5; Spectrin beta chain brain 2; Spectrin beta non-erythrocytic 2; Spectrin non-erythroid beta chain 2; Spinocerebellar ataxia 5; SPTBN2

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  SPTBN2

• Gene ID:

  6712

• NCBI Gene ID:

  26915

• UniProt:

  O15020

• Cellular Locus:

  Cytoskeleton

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant human SPTBN2 fragment (aa356-475) (exact sequence is proprietary)

• Target Antigen:

  Spectrin Beta-III

• Clonality:

  Monoclonal

• Isotype:

  IgG3

• Clone:

  SPTBN2/1247

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Field of Research:

  Cytoskeleton

• Positive Control:

  HeLa cells. Pancreas or Liver.

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  246 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• References & Citations:

  Speicher, D.W. 1986. The present status of erythrocyte spectrin structure: the 106-residue repetitive structure is a basic feature of an entire class of proteins. J. Cell. Biochem. 30: 245-258._x000D_ _x000D_ Coelman, T.R., et al. J.S. 1989. Functional diversity among spectrin isoforms. Cell Motil. Cytoskeleton 12: 225-247._x000D_

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4