Goat anti-Luciferase Polyclonal antibody
CAT:
894-AB0131-200
Size:
600 µg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Goat anti-Luciferase Polyclonal antibody
- Description: Goat polyclonal antibody to Firefly luciferase. Luciferase is a widely used reporter protein to study gene expression. Luciferase catalyses a bioluminescent reaction which requires luciferin as a substrate, ATP and Mg2+. A cell extract containing luciferase mixed luciferin in the presence of ATP and Mg2+, results in a flash of light that decays rapidly and can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
- Specifications: In 293HEK cells transfected with cds plasmid detects a band of 62 kDa by Western blot.
- Product Name Alternative: Luc and Luciferin 4-monooxygenase antibody.
- CAS Number: 9007-83-4
- UNSPSC Description: Firefly Luciferase
- Volume: 200 µL
- Host: Goat
- Antigen Species: Firefly
- Reactivity: Photinus pyralis
- Immunogen: Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli.
- Target Antigen: Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli.
- Immunogen Type: Recombinant protein
- Target: Anti-Luciferase
- Clonality: Polyclonal
- Isotype: IgG
- Conjugation: Unconjugated
- Type: Primary
- Sequence: LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL
- Applications: WB
- Purification: Epitope affinity purified
- Concentration: 3 mg/mL
- Dilution: WB:1:500-1:2,000
- Form: Polyclonal antibody supplied as a 200 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
- Buffer: PBS, 20% glycerol and 0.05% sodium azide
- Storage Conditions: For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.