Anti-Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) (PDPN/1433), CF568 conjugate

Anti-Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) (PDPN/1433), CF568 conjugate

• Description: This antibody recognizes a muco-protein of 38-43 kDa, which is identified Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi'sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Aggrus; Glycoprotein 36 KD; Glycoprotein 36; gp36; GP38; GP40; HT1A1; hT1alpha1; hT1alpha2; Lung type I cell membrane associated glycoprotein; Lung type I cell membrane associated glycoprotein T1A 2; OTS8; PA2.26; Pdpn; Podoplanin; PSEC0003; PSEC0025; T1-alpha; T1A; TI1A; TIA2
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: PDPN
• Gene ID: 10630
• NCBI Gene ID: 468675
• UniProt: Q86YL7
• Cellular Locus: Mitochondrial|Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant human Podoplanin (PDPN) protein fragment (aa24-126) (exact sequence is proprietary)
• Target Antigen: Podoplanin
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: PDPN/1433
• Conjugation: CF568
• Disease: Tumor
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Cancer
• Positive Control: HeLa Cells. Angiosarcoma or Mesothelioma.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 38-43 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Immunofluorescence 0.5-1 ug/mL Western blotting 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years