Anti-Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) (PDPN/1433), CF647 conjugate

• Description:

  This antibody recognizes a muco-protein of 38-43 kDa, which is identified Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi'sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Aggrus; Glycoprotein 36 KD; Glycoprotein 36; gp36; GP38; GP40; HT1A1; hT1alpha1; hT1alpha2; Lung type I cell membrane associated glycoprotein; Lung type I cell membrane associated glycoprotein T1A 2; OTS8; PA2.26; Pdpn; Podoplanin; PSEC0003; PSEC0025; T1-alpha; T1A; TI1A; TIA2

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  PDPN

• Gene ID:

  10630

• NCBI Gene ID:

  468675

• UniProt:

  Q86YL7

• Cellular Locus:

  Mitochondrial|Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human Podoplanin (PDPN) protein fragment (aa24-126) (exact sequence is proprietary)

• Target Antigen:

  Podoplanin

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  PDPN/1433

• Conjugation:

  CF647

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cancer

• Positive Control:

  HeLa Cells. Angiosarcoma or Mesothelioma.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  38-43 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Immunofluorescence 0.5-1 ug/mL Western blotting 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4