Recombinant Mouse GM-CSF (CSF2)

Recombinant Mouse GM-CSF (CSF2)

• Description: GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the aminoterminal end. The sugar moiety is not required for the full spectrum of biological activities. Non-glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non- glycosylated protein. This protein is secreted together with other factors by T cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF.
• CAS Number: 9000-83-3
• Gene Name: CSF2
• Gene Aliases: GMCSF, Colony-stimulating factor, CSF, Csfgm, Csf2
  Recombinant Mouse Granulocyte Macrophage Colony Stimulating Factor (CSF2)
• Gene ID: 12981
• Accession Number: P01587
• Organism: Mouse
• Source: E. coli
• Concentration: <1.0 EU/μg of recombinant protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE
• Solubility: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Function: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells is ≤ 0.1 ng/mL, corresponding to a specific activity of ≥ 3.0 x 10^6 units/mg.
• Molecular Weight: 14
• Shipping Conditions: Ambient Temperature
• Storage Conditions: The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
• Appearance: Lyophilized Powder
• Formulation: Recombinant mouse GM-CSF was lyophilized from 0.2 μm filtered PBS solution, pH 7.0.