Recombinant Human M-CSF (CSF1)
CAT:
171-Z100659
Size:
1.0 mg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No


Recombinant Human M-CSF (CSF1)
Description:
M-CSF is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. After cell activation, B cells and T cells and also a number of tumor cell lines are capable also of synthesizing this factor. M-CSF has been found to be synthesized by uterine epithelial cells in vivo. The M-CSF receptor is identical with the proto-oncogene fms. It has been renamed CD115. The receptor is a transmembrane protein with an extracellular ligand-binding domain of 512 amino acids, an intramembrane domain of 25 amino acids, and a cytoplasmic domain of 435 amino acids encoding a tyrosine kinase. Human M-CSF is active in mouse and rat cells. The murine factor is active in rat cells but inactive in human cells. M-CSF is a specific factor in that the proliferation inducing activity is more or less restricted to the macrophage lineage. M-CSF is a potent stimulator of functional activities of monocytes.Gene Name:
CSF1Gene Aliases:
Macrophage colony-stimulating factor 1, CSF-1, MCSF, MCSF, Lanimostim
Recombinant Human Macrophage Colony Stimulating Factor (CSF1)Gene ID:
1435Accession Number:
P09603Organism:
HumanSource:
E. coliConcentration:
<1.0 EU/μg of recombinant protein as determined by the LAL method.Purity:
>95% as determined by SDS-PAGESolubility:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.Function:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of M-NFS-60 cells is < 1.0 ng/mL, corresponding to a specific activity of > 1.0 x 10^6 units/mg.Molecular Weight:
18.5Shipping Conditions:
Ambient TemperatureStorage Conditions:
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.Appearance:
Lyophilized PowderFormulation:
Recombinant M-CSF was lyophilized from a 0.2 μm filtered PBS solution pH 7.0 containing 1% HSA, 3% mannitol.CAS Number:
9000-83-3