Recombinant Human Midkine (NEGF2)

• Description :

  Midkine and its functionally-related protein Pleiotrophin (PTN), are heparin-binding neurotrophic factors that signal through the same receptor. Midkine is involved in a number of biological processes including the migration of neutrophils, inflammatory leukocytes, macrophages, neuronal cells, and osteoclasts. Midkine is a basic, non-glycosylated polypeptide that contains five intra-chain disulfide bonds. There is 87% identity between the human and murine Midkine and approximately 50% identity between human Midkine and human PTN with the conservation of all 10 cysteines.

• Gene Name :

  MDK

• Gene Aliases :

  MK, NEGF-2

• Gene ID :

  4192

• Accession Number :

  P21741

• Organism :

  Human

• Source :

  E. coli

• Concentration :

  < 1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity :

  >95% as determined by SDS-PAGE

• Solubility :

  Reconstitute at 0.1 mg/ml in sterile PBS

• Function :

  The ED(50) was determined by the dose-dependent proliferation of AsPC-1 cells and was found to be <0.5ng/mL.

• Molecular Weight :

  14

• Shipping Conditions :

  Ambient Temperature

• Storage Conditions :

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance :

  Lyophilized Powder

• Formulation :

  Lyophilized from a 0.2 µm filtered solution in PBS

• CAS Number :

  9000-83-3