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Human ADP (Adiponectin) ELISA Kit

CAT: 0855-ELK1233-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1233-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADP in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
UniProt
Q15848
Reactivity
Human
Field of Research
Metabolic pathway; Infection immunity; Cardiovascular biology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADP in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
30 ng/mL
Sample Type
Serum, plasma
Detection Range
0.47-30 ng/mL
Sensitivity
75 pg/mL

UniProtKB · Q15848

Adiponectin

ADIPO_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q15848
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ADIPOQ
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q58EX9
Protein keywords

Technical term

3D-structureDirect protein sequencingPharmaceuticalProteomics identificationReference proteome

Domain

CollagenRepeatSignal

Disease

Diabetes mellitusDisease variantObesity

PTM

Disulfide bondGlycoproteinHydroxylation

Molecular function

Hormone

Cellular component

Secreted

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