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Cattle ADP (Adiponectin) ELISA Kit

CAT: 0855-ELK5893-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5893-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle ADP in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
UniProt
Q3Y5Z3
Reactivity
Cattle
Field of Research
Metabolic pathway; Infection immunity; Cardiovascular biology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle ADP in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
50 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.79-50 ng/mL
Sensitivity
0.34 ng/mL

UniProtKB · Q3Y5Z3

Adiponectin

ADIPO_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
Q3Y5Z3
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ADIPOQ
Protein existence
1: Evidence at protein level
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A5D7A8, Q95MQ4
Protein keywords

Domain

CollagenRepeatSignal

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinHydroxylation

Molecular function

Hormone

Cellular component

Secreted

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