Adiponectin ELISA Kit (Human)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (ng/mL) 13.8 37.2 67.3 13.1 36.8 65.7 St. Dev. 0.39 1.34 3.03 0.77 2.5 4.66 %CV 2.82 3.60 4.50 5.87 6.79 7.09 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (ng/mL) 13.8 37.2 67.3 13.1 36.8 65.7 Mean (ng/mL) 13.8 37.2 67.3 13.1 36.8 65.7 St. Dev. 0.39 1.34 3.03 0.77 2.5 4.66 St. Dev. 0.39 1.34 3.03 0.77 2.5 4.66 %CV 2.82 3.60 4.50 5.87 6.79 7.09 %CV 2.82 3.60 4.50 5.87 6.79 7.09

• Gene Name:

  Adiponectin, C1Q and collagen domain containing

• Gene Aliases:

  30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP30, ADIPO_HUMAN, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, Adipoq, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPQTL1, ADPN, apM-1, APM1, C1q and collagen domain-containing protein, GBP28, Gelatin binding protein, Gelatin binding protein 28, Gelatin-binding protein

• Gene ID:

  9370

• Accession Number:

  NP_001171271.1

• Reactivity:

  Human

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: E19-N244

• Target:

  Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226 (19-244) amino acids with the molecular mass of 36KDa after glycosylation.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's human Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Adiponectin has been precoated onto 96-well plates. Standards (NS0, E19-N244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Adiponectin amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates, serum, plasma (heparin, EDTA), tissue homogenates and urine

• Detection Range:

  1.56 ng/ml - 100 ng/ml

• Sensitivity:

  <60 pg/ml

• Homology:

  No detectable cross-reactivity with any other cytokine

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant human total Adiponectin

• NCBI Gene Symbol:

  ADIPOQ

• Protein Name:

  Adiponectin

• Gene Name URL:

  ADIPOQ

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001177800.1