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Human EIF4A2 (Eukaryotic Translation Initiation Factor 4A2) ELISA Kit

CAT: 0855-ELK4441-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4441-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EIF4A2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EIF4A2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EIF4A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EIF4A2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
EIF4A; BM-010; DDX2B; EIF4F; ATP-dependent RNA helicase eIF4A-2
UniProt
Q14240
Reactivity
Human
Field of Research
Developmental science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EIF4A2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EIF4A2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EIF4A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EIF4A2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.062 ng/mL

UniProtKB · Q14240

Eukaryotic initiation factor 4A-II

IF4A2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q14240
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EIF4A2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
eIF-4A-II, eIF4A-II
EC number
3.6.4.13
Processing
—
Secondary accessions
D3DNU9, Q53XJ6, Q96B90, Q96EA8
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Disease

Disease variantIntellectual disability

Molecular function

HelicaseHydrolaseInitiation factorRNA-binding

Biological process

Host-virus interactionProtein biosynthesis

PTM

Phosphoprotein

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