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Rat eIF4E (eukaryotic initiation factor 4E) ELISA Kit

CAT: 0855-ELK8552-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK8552-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat eIF4E. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat eIF4E. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat eIF4E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat eIF4E in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CBP; eIF 4E; eIF 4F 25 kDa subunit; EIF4E; EIF4E1; EIF4EL1; EIF4F
UniProt
P63074
Reactivity
Rat
Field of Research
Breast Cancer
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat eIF4E. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat eIF4E. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat eIF4E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat eIF4E in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
6000 pg/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
93.75-6000 pg/mL
Sensitivity
26.14 pg/mL

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