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Human EIF2A (Eukaryotic Translation Initiation Factor 2 subunit alpha) ELISA Kit

CAT: 0855-ELK0541-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK0541-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EIF2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EIF2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EIF2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EIF2A in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Eukaryotic translation initiation factor 2 subunit alpha (eIF-2-alpha; eIF-2A; eIF-2alpha)
UniProt
Q9BY44
Reactivity
Human
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EIF2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EIF2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EIF2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EIF2A in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
5000 pg/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
78.13-5000 pg/mL
Sensitivity
32.4 pg/mL

UniProtKB · Q9BY44

Eukaryotic translation initiation factor 2A

EIF2A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9BY44
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EIF2A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
eIF-2A
EC number
—
Processing
—
Secondary accessions
A8MPS6, B4DF96, B4DQ14, D3DNI9, Q5QTR2, Q7Z4E9, Q8NFM1, Q96EW9, Q96K81
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Domain

Coiled coilRepeatWD repeat

Molecular function

Initiation factor

Biological process

Protein biosynthesisTranslation regulation

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