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Human CSN3 (Casein Kappa) Microsample ELISA Kit

CAT: 0855-ELK4819MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4819MS-01Size:48 Tests
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Product Name Alternative
CSN10; CSNk; KCA; CASK
UniProt
P07498
Reactivity
Human
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CSN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CSN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CSN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CSN3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
200 ng/mL
Sample Type
Breast milk and other biological fluids
Detection Range
3.13-200 ng/mL
Sensitivity
1.34 ng/mL

UniProtKB · P07498

Kappa-casein

CASK_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P07498
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CSN3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q13575
Protein keywords

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

Milk protein

Cellular component

Secreted

Domain

Signal

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