Products for Research Use Only
RegisterLogin

Cattle ITGb2 (Integrin Beta 2) ELISA Kit

CAT: 0855-ELK9206-01Size: 48 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0855-ELK9206-01Size:48 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle ITGb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle ITGb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle ITGb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle ITGb2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CD18; ITG-B2; LAD; LFA1; MAC1; LCAMB; MF17; LFAb; Complement Component 3 Receptor 3 And 4 Subunit; Lymphocyte Function-Associated Antigen 1; Macrophage Antigen 1
UniProt
P32592
Reactivity
Cattle
Field of Research
Signal transduction; CD & Adhesion molecule; Tumor immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle ITGb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle ITGb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle ITGb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle ITGb2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.054 ng/mL

UniProtKB · P32592

Integrin beta-2

ITB2_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
P32592
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ITGB2
Protein existence
1: Evidence at protein level
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Ligand

CalciumMagnesiumMetal-binding

Biological process

Cell adhesionPhagocytosis

Cellular component

Cell membraneMembrane

Disease

Disease variant

PTM

Disulfide bondGlycoproteinPhosphoproteinPyrrolidone carboxylic acid

Domain

EGF-like domainRepeatSignalTransmembraneTransmembrane helix

Molecular function

IntegrinReceptor

Technical term

Reference proteome

Related Products

CatalogName
ELK9401-01Mouse ITGb2 (Integrin Beta 2) ELISA Kit
ELK4937-01Human ITGb2 (Integrin Beta 2) ELISA Kit
ELK9401MS-01Mouse ITGb2 (Integrin Beta 2) Microsample ELISA Kit
ELK4937MS-01Human ITGb2 (Integrin Beta 2) Microsample ELISA Kit
ELK7064-01Cattle DEFb2 (Defensin Beta 2) ELISA Kit
ELK0528-01Cattle β2-MG (Beta-2-Microglobulin) ELISA Kit
ELK0536-01Cat ITGb1 (Integrin Beta 1) ELISA Kit
ELK4242-01Mouse ITGb3 (Integrin Beta 3) ELISA Kit
ELK5301-01Mouse ITGb1 (Integrin Beta 1) ELISA Kit
ELK8442-01Rat ITGb3 (Integrin Beta 3) ELISA Kit

Alternative Products

CatalogName
216747ITGB2 (Integrin beta 2)
MBS2010494-01Recombinant Integrin Beta 2 (ITGb2)
abx001785-01Integrin Beta 2 (ITGB2) Antibody
abx421198-01Integrin Beta-2 (ITGB2) Antibody
abx617252Integrin Beta 2 (ITGB2) Peptide
QY-E60169Cow Integrin Beta 2 (ITGB2)

Popular Products