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Cattle ACTb (Actin Beta) ELISA Kit

CAT: 0855-ELK11229-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK11229-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
UniProt
P60712
Reactivity
Cattle
Field of Research
Signal transduction; Developmental science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle ACTb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle ACTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle ACTb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle ACTb in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.57 ng/mL

UniProtKB · P60712

Actin, cytoplasmic 1

ACTB_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
P60712
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ACTB
Protein existence
1: Evidence at protein level
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
—
EC number
3.6.4.-
Processing
—
Secondary accessions
A5D961, A5PKA1, P02570, P70514, P99021, Q11211, Q3SZD2, Q64316, Q8MIJ0
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

AcetylationMethylationOxidationUbl conjugation

Ligand

ATP-bindingNucleotide-binding

Cellular component

CytoplasmCytoskeletonNucleus

Molecular function

Hydrolase

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