SingleFlowEx CD24 Alexa Fluor® 700

SingleFlowEx CD24 Alexa Fluor® 700

• Description: SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
• Certification: RUO, GMP
• Clone: SN3
• Validated Applications: FC
• Concentration: 0.080 mg/mL
• Format: Alexa Fluor® 700
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Components: 1 vial of monoclonal antibody reagent. The content (1 mL) is sufficient for min. 100 Tests.
• References & Citations: *Všianská P, Říhová L, Varmužová T, Suská R, Kryukov F, Mikulášová A, Kupská R, Penka M, Pour L, Adam Z, Hájek R: Analysis of B-cell subpopulations in monoclonal gammopathies. Clin Lymphoma Myeloma Leuk. 2015 Apr15(4):e61-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/25578543 ,*Maliar A, Servais C, Waks T, Chmielewski M, Lavy R, Altevogt P, Abken H, Eshhar Z: Redirected T cells that target pancreatic adenocarcinoma antigens eliminate tumors and metastases in mice. Gastroenterology. 2012 Nov143(5):1375-1384, URL: http://www.ncbi.nlm.nih.gov/pubmed?term=maliar%20a%20servais%20c%20waks%20t ,*Fukukawa T, Matsuzaki H, Haruta Y, Hara H, Seon BK: New monoclonal antibodies SN3, SN3a, and SN3b directed to sialic acid of glycoprotein on human non-T leukemia cells. Exp Hematol. 1986 Oct14(9):850-5., URL: https://pubmed.ncbi.nlm.nih.gov/3489635/,*Barcos M, Pollard C, Fukukawa T, Seon BK: Follicular mantle zone cell subpopulations detected by monoclonal antibody SN3. Hematol Oncol. 1986 Oct-Dec4(4):251-9., URL: https://pubmed.ncbi.nlm.nih.gov/3493967/,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL: ,*Solvason N, Kearney JF. The human fetal omentum: a site of B cell generation. J Exp Med. 1992 Feb 1175(2):397-404., URL: https://pubmed.ncbi.nlm.nih.gov/1370683/,*Fischer GF, Majdic O, Gadd S, Knapp W. Signal transduction in lymphocytic and myeloid cells via CD24, a new member of phosphoinositol-anchored membrane molecules. J Immunol. 1990 Jan 15144(2):638-41., URL: https://pubmed.ncbi.nlm.nih.gov/2153173/,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL: ,*Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan94(1):23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353,*Use of this product has not been published., URL: ,
• Other References: *Runz S, Mierke CT, Joumaa S, Behrens J, Fabry B, Altevogt P: CD24 induces localization of beta1 integrin to lipid raft domains. Biochem Biophys Res Commun. 2008 Jan 4365(1):35-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/17980703?ordin,*Chou YY, Jeng YM, Lee TT, Hu FC, Kao HL, Lin WC, Lai PL, Hu RH, Yuan RH: Cytoplasmic CD24 expression is a novel prognostic factor in diffuse-type gastric adenocarcinoma. Ann Surg Oncol. 2007 Oct14(10):2748-58., URL: https://www.ncbi.nlm.nih.gov/pubmed/17680316?ordin,*Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P: CD24 is a marker of exosomes secreted into urine and amniotic fluid. Kidney Int. 2007 Nov72(9):1095-102., URL: https://www.ncbi.nlm.nih.gov/pubmed/17700640?ordin,*Schabath H, Runz S, Joumaa S, Altevogt P: CD24 affects CXCR4 function in pre-B lymphocytes and breast carcinoma cells. J Cell Sci. 2006 Jan 15119(Pt 2):314-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/16390867?ordin,*Suzuki T, Kiyokawa N, Taguchi T, Sekino T, Katagiri YU, Fujimoto J: CD24 induces apoptosis in human B cells via the glycolipid-enriched membrane domains/rafts-mediated signaling system. J Immunol. 2001 May 1166(9):5567-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/11313396?ordin,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.