SingleFlowEx CD38 Alexa Fluor® 700

SingleFlowEx CD38 Alexa Fluor® 700

• Description: SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
• Certification: RUO, GMP
• Clone: HIT2
• Validated Applications: FC
• Concentration: 0.040 mg/mL
• Format: Alexa Fluor® 700
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Components: 1 vial of monoclonal antibody reagent. The content (1 mL) is sufficient for min. 100 Tests.
• References & Citations: *Leukocyte Typing III., McMichael AJ et al (Eds.), Oxford University Press (1987)., URL: ,*Rozková D, Novotná L, Pytlík R, Hochová I, Kozák T, Bartůnková J, Spísek R: Toll-like receptors on B-CLL cells: expression and functional consequences of their stimulation. Int J Cancer. 2010 Mar 1126(5):1132-43., URL: https://www.ncbi.nlm.nih.gov/pubmed/19685493,*Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15109(6):2545-52., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1852199/,*Všianská P, Říhová L, Varmužová T, Suská R, Kryukov F, Mikulášová A, Kupská R, Penka M, Pour L, Adam Z, Hájek R: Analysis of B-cell subpopulations in monoclonal gammopathies. Clin Lymphoma Myeloma Leuk. 2015 Apr15(4):e61-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/25578543 ,*Ratuszny D, Skripuletz T, Wegner F, Groß M, Falk C, Jacobs R, Ruschulte H, Stangel M, Sühs KW: Case report: Daratumumab in a patient with severe refractory anti-NMDA receptor encephalitis. Front Neurol. 2020 Dec 2211:602102., URL: https://pubmed.ncbi.nlm.nih.gov/33414761/,*Kralickova P, Milota T, Litzman J, Malkusova I, Jilek D, Petanova J, Vidlakova J, Zimulova A, Fronkova E, Svaton M, Kanderova V, Bloomfeld M, Parackova Z, Klocperk A, Haviger J, Kalina T, Sediva A: CVID-associated tumors: Czech nationwide study focused on epidemiology, immunology, and genetic background in a cohort of patients with CVID. Frontiers in Immunology 2019, 9:3135, URL: ,
• Other References: *Cakir-Kiefer C, Muller-Steffner H, Oppenheimer N, Schuber F: Kinetic competence of the cADP-ribose-CD38 complex as an intermediate in the CD38/NAD+ glycohydrolase-catalysed reactions: implication for CD38 signalling. Biochem J. 2001 Sep 1358(Pt 2):399-406., URL: https://www.ncbi.nlm.nih.gov/pubmed/11513738?ordin,*Lund FE, Muller-Steffner H, Romero-Ramirez H, Moreno-García ME, Partida-Sánchez S, Makris M, Oppenheimer NJ, Santos-Argumedo L, Schuber F: CD38 induces apoptosis of a murine pro-B leukemic cell line by a tyrosine kinase-dependent but ADP-ribosyl cyclase- and NAD glycohydrolase-independent mechanism. Int Immunol. 2006 Jul18(7):1029-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/16720618?ordin,*Partida-Sanchez S, Gasser A, Fliegert R, Siebrands CC, Dammermann W, Shi G, Mousseau BJ, Sumoza-Toledo A, Bhagat H, Walseth TF, Guse AH, Lund FE. Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp-ribose, the major product generated by the CD38 enzyme reaction. J Immunol. 2007 Dec 1179(11):7827-39., URL: https://www.ncbi.nlm.nih.gov/pubmed/18025229?ordin,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.