SingleFlowEx CD3 Alexa Fluor® 700

SingleFlowEx CD3 Alexa Fluor® 700

• Description: SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
• Certification: RUO, GMP
• Clone: UCHT1
• Validated Applications: FC
• Concentration: 0.10 mg/mL
• Format: Alexa Fluor® 700
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Components: 1 vial of monoclonal antibody reagent. The content (1 mL) is sufficient for min. 100 Tests.
• References & Citations: *Rieux-Laucat F, Hivroz C, Lim A, Mateo V, Pellier I, Selz F, Fischer A, Le Deist F: Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency. N Engl J Med. 2006 May 4354(18):1913-21., URL: http://www.ncbi.nlm.nih.gov/pubmed/16672702?itool=,*Arnett KL, Harrison SC, Wiley DC: Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment. Proc Natl Acad Sci U S A. 2004 Nov 16101(46):16268-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/15534202?itool,*Torres PS, Alcover A, Zapata DA, Arnaud J, Pacheco A, Martín-Fernández JM, Villasevil EM, Sanal O, Regueiro JR: TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J Immunol. 2003 Jun 15170(12):5947-55., URL: https://www.ncbi.nlm.nih.gov/pubmed/12794121?itool,*le Gouvello S, Manceau V, Sobel A: Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes. J Immunol. 1998 Aug 1161(3):1113-22. , URL: https://www.ncbi.nlm.nih.gov/pubmed/9686569?itool=,*Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15106(10):3567-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/16046525?itool,*Demedts IK, Brusselle GG, Vermaelen KY, Pauwels RA: Identification and characterization of human pulmonary dendritic cells. Am J Respir Cell Mol Biol. 2005 Mar32(3):177-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/15576669?itool,*Fisch P, Malkovsky M, Braakman E, Sturm E, Bolhuis RL, Prieve A, Sosman JA, Lam VA, Sondel PM: Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis. J Exp Med. 1990 May 1171(5):1567-79., URL: https://www.ncbi.nlm.nih.gov/pubmed/2185329?ordina,*Siegers GM, Swamy M, Fernández-Malavé E, Minguet S, Rathmann S, Guardo AC, Pérez-Flores V, Regueiro JR, Alarcón B, Fisch P, Schamel WW: Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies. J Exp Med. 2007 Oct 29204(11):2537-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/17923503?ordin,*Barclay, Brown et al.: The Leukocyte Antigen FactsBook, 2nd edition, Harcourt Brace & Company, London, (1997)., URL: ,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,*Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987)., URL: ,*Garson JA, Beverley PC, Coakham HB, Harper EI: Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Nature. 1982 Jul 22298(5872):375-7., URL: https://pubmed.ncbi.nlm.nih.gov/6178042/,*Dixon JF, Law JL, Favero JJ: Activation of human T lymphocytes by crosslinking of anti-CD3 monoclonal antibodies. J Leukoc Biol. 1989 Sep46(3):214-20., URL: https://pubmed.ncbi.nlm.nih.gov/2788205/,*Verwilghen J, Baroja ML, Van Vaeck F, Van Damme J, Ceuppens JL: Differences in the stimulating capacity of immobilized anti-CD3 monoclonal antibodies: Variable dependence on interleukin-1 as a helper signal for T-cell activation. Immunology 1991 Feb72(2):269-76., URL: https://pubmed.ncbi.nlm.nih.gov/1826671/,*Harper KS, Strefford JC, Cragg MS, Glennie MJ, Williams AP, French RR: Upregulation of FcγRIIb on monocytes is necessary to promote the superagonist activity of TGN1412. Blood 2015 Jan 1125(1):102-10., URL: https://pubmed.ncbi.nlm.nih.gov/25395427/,*Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug20(8):1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/,*Use of this product has not been published., URL: ,
• Other References: *Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9279(28):28827-30., URL: https://pubmed.ncbi.nlm.nih.gov/15084594/,*Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May7(5):490-5., URL: https://pubmed.ncbi.nlm.nih.gov/16670682/,*Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb24(2):133-9., URL: https://pubmed.ncbi.nlm.nih.gov/16473826/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.