TUNEL In Situ Apoptosis Kit (HRP-DAB Method)

• Type:

  Apoptosis / DNA Fragmentation

• Detection Method:

  Colorimetric method

• Assay Principle:

  When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP) . Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis.

• Assay Performance Time:

  3 Hours

• Sample Type:

  Paraffin section; frozen section; cell slide

• Shipping Conditions:

  Ice Bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://link.springer.com/article/10.1186/s12943-024-02074-z

• Citation 02:

  https://pubs.acs.org/doi/abs/10.1021/acsnano.4c07085

• Citation 03:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202407493

• Citation 04:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202401731

• Citation 05:

  https://www.sciencedirect.com/science/article/pii/s1385894724061448

• Citation 06:

  https://www.sciencedirect.com/science/article/pii/s2213231724001058

• Citation 07:

  https://aacrjournals.org/cancerres/article/doi/10.1158/0008-5472.can-23-1796/733406

• Citation 08:

  https://www.sciencedirect.com/science/article/pii/s2213231723001842

• Citation 09:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202301325

• Citation 10:

  https://www.sciencedirect.com/science/article/pii/s0144861724008221

• Citation 11:

  https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-024-02876-9

• Citation 12:

  https://link.springer.com/article/10.1186/s12916-023-03243-5

• Detection Instrument:

  Light microscope