One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

• Detection Method:

  Fluorometric method

• Assay Principle:

  When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

• Sample Type:

  Paraffin section; frozen section; cell slide

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://www.nature.com/articles/s41588-023-01528-2

• Citation 02:

  https://www.nature.com/articles/s41368-022-00198-w

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/S2095927323006953

• Citation 04:

  https://www.sciencedirect.com/science/article/pii/S2452199X24001488

• Citation 05:

  https://pubs.acs.org/doi/abs/10.1021/acsnano.3c08485

• Citation 06:

  https://www.nature.com/articles/s41467-023-41782-w

• Citation 07:

  https://academic.oup.com/nar/advance-article-abstract/doi/10.1093/nar/gkae274/7650606

• Citation 08:

  https://www.nature.com/articles/s41467-024-44687-4

• Citation 09:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202302527

• Citation 10:

  https://www.sciencedirect.com/science/article/pii/S1385894723068018

• Citation 11:

  https://pmc.ncbi.nlm.nih.gov/articles/PMC12120566/

• Citation 12:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202406500

• Detection Instrument:

  Fluorescence Microscope