One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)

• Detection Method:

  Fluorometric method

• Assay Principle:

  When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

• Sample Type:

  Paraffin section; frozen section; cell slide

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://www.cell.com/cell/pdf/S0092-8674(23)01176-5.pdf

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S2452199X24005309

• Citation 03:

  https://www.nature.com/articles/s41467-023-42972-2

• Citation 04:

  https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/advs.202417605

• Citation 05:

  https://www.nature.com/articles/s41467-025-58805-3

• Citation 06:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/agt2.70086

• Citation 07:

  https://www.sciencedirect.com/science/article/pii/S1385894724042232

• Citation 08:

  https://www.tandfonline.com/doi/abs/10.1080/19490976.2025.2489072

• Citation 09:

  https://link.springer.com/article/10.1186/s40824-023-00377-8

• Citation 10:

  https://link.springer.com/article/10.1186/s12951-024-02735-7

• Citation 11:

  https://www.sciencedirect.com/science/article/pii/S1043661824005012

• Citation 12:

  https://www.sciencedirect.com/science/article/pii/S004896972404508X

• Detection Instrument:

  Fluorescence Microscope