One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 647)

• Type:

  Apoptosis / DNA Fragmentation

• Detection Method:

  Fluorometric method

• Assay Principle:

  When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

• Assay Performance Time:

  3 Hours

• Sample Type:

  Paraffin section; frozen section; cell slide

• Shipping Conditions:

  Ice Bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://link.springer.com/article/10.1007/s00401-023-02675-w

• Citation 02:

  https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-024-02780-2

• Citation 03:

  https://www.embopress.org/doi/abs/10.1038/s44319-024-00109-6

• Citation 04:

  https://www.sciencedirect.com/science/article/pii/s0944711323006487

• Citation 05:

  https://academic.oup.com/stmcls/advance-article-abstract/doi/10.1093/stmcls/sxad037/7174085

• Citation 06:

  https://www.mdpi.com/2073-4409/13/13/1074

• Citation 07:

  https://www.hindawi.com/journals/jir/2022/4589182/

• Citation 08:

  https://www.jstage.jst.go.jp/article/jts/47/9/47_349/_article/-char/ja/

• Detection Instrument:

  Fluorescence Microscope