Rat bFGF Protein

• Background :

  Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic over-expression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions.

• Description :

  Rat bFGF protein, expressed in E. coli

• Product Name Alternative :

  Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB

• Gene ID :

  54250

• Accession Number :

  P13109

• Tag :

  BFGF

• Type :

  Cytokines and proteins

• Applications :

  No tag

• Purity :

  > 95 % as determined by SDS-PAGE.

• Activity :

  Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.0015 ng/mL.The specific activity of recombinant Rat bFGF approximately >6.67 x 108 IU/mg

• Molecular Weight :

  16.2 kDa

• Precautions :

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Features & Benefits :

  Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.

• Shipping Conditions :

  Gel pack with blue ice.

• Storage Conditions :

  Lyophilized Rat bFGF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

• Protein Length :

  The recombinant Rat bFGF protein consists of 145 amino acids (N-Met) and has a predicted molecular mass of 16.2 KD. The apparent molecular mass of the Rat FGF2 is approximately 16.2 KD in SDS-PAGE under reducing conditions due to glycosylation.

• Formulation :

  Lyophilized from sterile 20 mM Tris,150 mM NaCl, pH8.0.

• Applications Notes :

  Rat

• Other Product Names :

  Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB

• Recommended Usage :

  Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Rat bFGF protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.

• Preparation :

  E. coli

• Accession Number URL :

  https://www.uniprot.org/uniprot/P13109

• AA Sequence :

  Amino acid sequence derived from Rat FGF2 isoform (P13109) (Ala11-Ser154) was expressed.