Mouse bFGF Protein

• Background:

  Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI) . bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.

• Description:

  Mouse bFGF protein, expressed in E. coli

• Product Name Alternative:

  BFGF; FGF-2; FGFB; HBGF-2; Fibroblast growth factor 2; FGF2

• Gene ID:

  14173

• Accession Number:

  P15655

• Tag:

  BFGF

• Type:

  Cytokines and proteins

• Applications:

  No tag

• Purity:

  > 98 % as determined by SDS-PAGE

• Activity:

  Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.11 ng/mL. The specific activity of recombinant Mouse bFGF is approximately >9.09 x 106 IU/mg

• Molecular Weight:

  18.5 kDa

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Features & Benefits:

  Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Lyophilized Mouse bFGF protein should be stored desiccated below -20°C.Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

• Protein Length:

  The recombinant Mouse bFGF protein consists of 146 amino acids and predicts a molecular mass of 18.5 kDa

• Formulation:

  Lyophilized from sterile 150 mM NaCl 20mM PB , pH 7.4.

• Applications Notes:

  Mouse

• Other Product Names:

  BFGF; FGF-2; FGFB; HBGF-2; Fibroblast growth factor 2; FGF2

• Recommended Usage:

  Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse bFGF protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Preparation:

  E. coli

• Accession Number URL:

  http://www.uniprot.org/uniprot/P15655

• AA Sequence:

  Amino acid sequence derived from Mouse bFGF soluble form (NP_15655) (Pro10-Ser154) was expressed, with an initial Met at the N-terminus.