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Human bFGF Protein

CAT: 0749-PRP1010-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0749-PRP1010-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI) . bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.
Description
Human bFGF protein, expressed in E. coli
Product Name Alternative
BFGF; FGF-2; FGFB; HBGF-2; Fibroblast growth factor 2; FGF2
Gene ID
2247
Accession Number
P09038
Tag
BFGF
Type
Cytokines and proteins
Applications
No tag
Purity
> 95 % as determined by SDS-PAGE
Activity
Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.0056 ng/mL. The specific activity of recombinant Human bFGF is approximately >1.79 x 108 IU/mg
Molecular Weight
17.3 kDa
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Lyophilized Human bFGF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Protein Length
The recombinant human bFGF protein consists of 158 amino acids and predicts a molecular mass of 17.3 kDa.
Formulation
Lyophilized from sterile 150mM NaCl 20mM Tris , pH 7.5.
Applications Notes
Human
Other Product Names
BFGF; FGF-2; FGFB; HBGF-2; Fibroblast growth factor 2; FGF2
Recommended Usage
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human bFGF protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Preparation
E. coli
Accession Number URL
http://www.uniprot.org/uniprot/P09038
AA Sequence
Amino acid sequence derived from human bFGF soluble form (NP_09038) (Ala135-Ser288) was expressed, with an initial Met at the N-terminus.

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