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Recombinant Mouse FGF2/bFGF Protein

CAT: 1676-PR00025Size: 10μg/50μg/500μg/1mgDry Ice: NoHazardous: No
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CAT#:1676-PR00025Size:10μg/50μg/500μg/1mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
FGF basic is one of 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine, and sheep FGF basic. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity. Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis is reminiscent of a variety of pathological conditions.
Product Name Alternative
Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; Fgf2; Fgf-2
Accession Number
P15655
Tag
Tag free
Source
E.coli
Endotoxin
<0.01 EU/μg as determined by LAL test.
Purity
>95% as determined by SDS-PAGE.
Form
Lyophilized
Reconstitution
Reconstitute the lyophilized protein in sterile water at a concentration of greater than 100 μg/mL.
Molecular Weight
17.15 KDa
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. Please minimize freeze-thaw cycles and use a manual defrost freezer.
Protein Length
Met1-Ser154
Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM PB,0.02% Tween 80,2.0% Sucrose,4.0% Manntiol, pH 7.0.
Species
Mouse