Human GM-CSF Protein

CAT: 0749-PRP1016-01Size: 5 µgDry Ice: NoHazardous: No
CAT#:0749-PRP1016-01Size:5 µg
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Background
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. GM-CSF is used therapeutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia (AML) .
Description
Human GM-CSF protein, expressed in E. coli
Product Name Alternative
GM-CSF; GMCSF; Granulocyte-macrophage colony-stimulating factor; CSF2
Gene ID
1437
Accession Number
P04141
Tag
GM-CSF
Type
Cytokines and proteins
Applications
No tag
Purity
> 98 % as determined by SDS-PAGE
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemia cells. The ED50 for this effect is 0.05-0.5 ng/mL.
Molecular Weight
14 kDa
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Lyophilized Human GM-CSF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Protein Length
The recombinant human GM-CSF consists of 128 amino acids and has a predicted molecular mass of 14 kDa.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Applications Notes
Human
Other Product Names
GM-CSF; GMCSF; Granulocyte-macrophage colony-stimulating factor; CSF2
Recommended Usage
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human GM-CSF protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Preparation
E. coli
Accession Number URL
https://www.uniprot.org/uniprot/P04141
AA Sequence
Amino acid sequence derived from human GM-CSF isoform (P04141-1) (Ala18-Glu144) was expressed.

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