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Mouse GM-CSF Protein

CAT: 0749-PRP1077-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0749-PRP1077-01Size:1 mg
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Background
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli.Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines
Description
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli.
Product Name Alternative
CSF; CSF2; CSF-2; GMCSF; GM-CSF; colony stimulating factor 2 (granulocyte-macrophage) ; granulocyte-macrophage colony-stimulating factor; Colony-stimulating factor; GMCSF, granulocyte-macrophage colony stimulating factor; molgramostin; Sargramostim, MGC131935; MGC138897; Molgramostim
Gene ID
12981
Accession Number
P01587
Tag
GM-CSF
Type
Cytokines and proteins
Applications
No tag
Purity
> 96 % as determined by SDS-PAGE
Activity
Measured in a cell proliferation assay using FDC-P1 cells.The ED50 for this effect is 1.203 ng/mL.
Molecular Weight
14.2 kDa
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin: <1 EU per 1 μg of the protein by the LAL method.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
The recombinant Mouse GM-CSF protein product should be stored desiccated below -20°C.The protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles
Protein Length
The recombinant Mouse GM-CSF consists of 124 amino acids and predicts a molecular mass of 14.2 kDa
Formulation
The protein was liquid in from sterile 20 mM Tris, 150 mM NaCl ,5% Glycerol, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Applications Notes
Mouse
Other Product Names
CSF; CSF2; CSF-2; GMCSF; GM-CSF; colony stimulating factor 2 (granulocyte-macrophage) ; granulocyte-macrophage colony-stimulating factor; Colony-stimulating factor; GMCSF, granulocyte-macrophage colony stimulating factor; molgramostin; Sargramostim, MGC131935; MGC138897; Molgramostim
Recommended Usage
Always centrifuge tubes before opening. It is recommended to diluted the protein using the PBS buffer or Protein storage solution to working concentration
Preparation
E. coli
Accession Number URL
VEGFA - Vascular endothelial growth factor A, long form - Homo sapiens (Human) | UniProtKB | UniProt
AA Sequence
Amino acid sequence derived from Mouse GM-CSF (Ala18-Lys141) (P01587) was expressed

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