APAF1 Rabbit Polyclonal Antibody

APAF1 Rabbit Polyclonal Antibody

• Description: Apoptotic protease activating factor 1, also known as APAF1, is a human homolog of C. elegans CED-4 gene. This gene encodes a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. This protein contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. The precise mechanism for this reaction is still debated though work published by Guy Salvesen suggests that the apoptosome may induce caspase 9 dimerization and subsequent autocatalysis. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis.
• Product Name Alternative: APAF 1 antibody Apaf-1 antibody APAF_HUMAN antibody Apaf1 antibody Apoptotic peptidase activating factor 1 antibody Apoptotic protease activating factor 1 antibody Apoptotic protease activating factor antibody Apoptotic protease-activating factor 1 antibody CED 4 antibody CED4 antibody KIAA0413 antibody
• Abbreviation: APAF-1
• CAS Number: 9007-83-4
• Swiss Prot: O14727 Human, O88879 Mouse
• Cellular Locus: Cytoplasm
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Synthetic peptide within Human APAF1 aa 1,191-1240 / 1,248.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Rabbit polyclonal Antibody
• Applications: WB
• Positive Control: HepG2, THP-1
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:1000
• Purity: Immunogen affinity purified.
• Form: Liquid
• Buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 142 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: No