MLST8 Rabbit Polyclonal Antibody

Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

9007-83-4

G protein beta subunit like antibody G protein beta subunit-like antibody Gable antibody GbetaL antibody GBL antibody GBL protein antibody LST8 antibody LST8_HUMAN antibody Mammalian lethal with SEC13 protein 8 antibody MGC111011 antibody mLST8 antibody MTOR associated protein LST8 homolog (S. cerevisiae) antibody POP3 antibody POP3 homolog (S. pombe) antibody Protein GbetaL antibody Target of rapamycin complex subunit LST8 antibody TORC subunit LST8 antibody WAT1 antibody WAT1 homolog (S. pombe) antibody

Target of rapamycin complex subunit LST8 TORC subunit LST8 G protein beta subunit-like Gable Protein GbetaL Mammalian lethal with SEC13 protein 8 mLST8 MLST8 GBL LST8

Q9BVC4 Human, Q9DCJ1 Mouse, Q9Z2K5 Rat

Cytoplasm.

Recombinant protein within human MLST8 aa 1-250.

Non-conjugated

Rabbit polyclonal Antibody

WB, IF-Cell, IHC-P

C2C12 cell lysate, L6 cell lysate, mouse brain tissue lysate, human brain tissue lysate, rat brain tissue lysate, 293 cell lysate, MCF-7 cell lysate, PC-3M, rat heart tissue, mouse skeletal muscle tissue.

2 mg/mL

WB: 1:500-1:1,000 ;IF-Cell: 1:200 ;IHC-P: 1:800

Immunogen affinity purified.

Liquid

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Predicted band size: 36 kDa

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.