HLA F Recombinant Rabbit Monoclonal Antibody [JE54-04]

HLA F Recombinant Rabbit Monoclonal Antibody [JE54-04]

• Description: Non-classical major histocompatibility class Ib molecule postulated to play a role in immune surveillance, immune tolerance and inflammation. Functions in two forms, as a heterotrimeric complex with B2M/beta-2 microglobulin and a peptide (peptide-bound HLA-F-B2M) and as an open conformer (OC) devoid of peptide and B2M (peptide-free OC). In complex with B2M, presents non-canonical self-peptides carrying post-translational modifications, particularly phosphorylated self-peptides. Peptide-bound HLA-F-B2M acts as a ligand for LILRB1 inhibitory receptor, a major player in maternal-fetal tolerance. Peptide-free OC acts as a ligand for KIR3DS1 and KIR3DL2 receptors. Upon interaction with activating KIR3DS1 receptor on NK cells, triggers NK cell degranulation and anti-viral cytokine production. Through interaction with KIR3DL2 receptor, inhibits NK and T cell effector functions. May interact with other MHC class I OCs to cross-present exogenous viral, tumor or minor histompatibility antigens to cytotoxic CD8+ T cells, triggering effector and memory responses (PubMed:23851683). May play a role in inflammatory responses in the peripheral nervous system. Through interaction with KIR3DL2, may protect motor neurons from astrocyte-induced toxicity.
• Product Name Alternative: CDA12 antibody HLA 5.4 antibody HLA class I histocompatibility antigen, alpha chain F antibody HLA class I molecule antibody HLA F antigen antibody HLA-CDA12 antibody HLA-F antibody HLAF antibody HLAF_HUMAN antibody Leukocyte antigen F antibody MHC class I antigen F antibody
• Abbreviation: HLA-F
• CAS Number: 9000-83-3
• Swiss Prot: P30511 Human
• Cellular Locus: Lysosome membrane, early endosome membrane, cell membrane.
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Recombinant protein within Human HLA F aa 1-127 / 346.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P
• Positive Control: Human thymus tissue lysate, human spleen tissue lysate, human tonsil tissue, human spleen tissue.
• Concentration: 1 mg/mL
• Dilution: WB:1:500-1:2,000 ;IHC-P:1:50-1:200
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 39 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes