RHEB Recombinant Rabbit Monoclonal Antibody [JG37-12]

RHEB Recombinant Rabbit Monoclonal Antibody [JG37-12]

• Description: H-, K- and N-Ras represent the prototype members of a family of small G proteins which are frequently activated to an oncogenic state in a wide variety of human tumors. Activation is due to point mutations at position 12 or 61 within their coding sequence. Such mutations cause these proteins to be constitutively converted to their active GTP-bound rather than the inactive GDP-bound state. The related human R-Ras gene was initially cloned by low stringency hybridization methods. Position 38 or 87 mutants of R-Ras (analogous to positions 12 and 61 in H-Ras) have been shown to be capable of activating oncogenic function. Ras p21 in its active GTP binding state binds to Raf-1, resulting in activation of the MAP kinase signaling cascade. An additional member of the Ras family, Rheb (Ras-related GTP-binding protein), also interacts with Raf-1. This interaction is potentiated by growth factors and agents that increase cAMP levels.
• Product Name Alternative: Ras homolog enriched in brain 2, formerly antibody GTP binding protein Rheb antibody GTP-binding protein Rheb antibody MGC111559 antibody Ras homolog enriched in brain 2 antibody Ras homolog enriched in brain antibody RHEB 2 antibody Rheb antibody RHEB_HUMAN antibody RHEB2 antibody RHEB2, formerly antibody
• Abbreviation: RHEB, RHEB2
• CAS Number: 9000-83-3
• Swiss Prot: Q15382 Human, Q921J2 Mouse
• Cellular Locus: Cytoplasm. Golgi apparatus. Endoplasmic reticulum.
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant protein within Human RHEB aa 21-108 / 184.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
• Positive Control: Wild-type Raw264.7 whole cell lysate, mouse placenta tissue lysates, Neuro-2a, A431, SH-SY5Y, human liver tissue, human colon tissue, human fetal skeletal muscle tissue, Hela.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 20 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes