Thymidylate Synthase Recombinant Rabbit Monoclonal Antibody [JM33-20]

Thymidylate Synthase Recombinant Rabbit Monoclonal Antibody [JM33-20]

• Description: Thymidylate synthase (TS) (EC 2.1.1.45) is an enzyme that catalyzes the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP). Thymidine is one of the nucleotides in DNA. With inhibition of TS, an imbalance of deoxynucleotides and increased levels of dUMP arise. Both cause DNA damage. By means of reductive methylation, deoxyuridine monophosphate (dUMP) and N5,N10-methylene tetrahydrofolate are together used to form dTMP, yielding dihydrofolate as a secondary product. This provides the sole de novo pathway for production of dTMP and is the only enzyme in folate metabolism in which the 5,10-methylenetetrahydrofolate is oxidised during one-carbon transfer. The enzyme is essential for regulating the balanced supply of the 4 DNA precursors in normal DNA replication: defects in the enzyme activity affecting the regulation process cause various biological and genetic abnormalities, such as thymineless death.
• Product Name Alternative: d TMP synthase antibody EC 2.1.1.45 antibody HsT422 antibody MGC88736 antibody OTTHUMP00000162195 antibody Thymidylate synthase antibody Thymidylate synthetase antibody TMS antibody TS antibody TSase antibody Tyms antibody TYMS protein antibody Tyms thymidylate synthetase antibody TYSY_HUMAN antibody
• Abbreviation: TS, TSase, TYMS, OK/SW-cl.29
• CAS Number: 9000-83-3
• Swiss Prot: P04818 Human, P07607 Mouse, P45352 Rat
• Cellular Locus: Nucleus. Cytoplasm. Mitochondrion.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within Human Thymidylate Synthase aa 264-313 / 313.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, FC, IP
• Positive Control: Hela cell lysate, Jurkat cell lysate, Raji cell lysate, mouse thymus tissue lysate, mouse colon tissue, human tonsil tissue, rat bone tissue, Hela.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 36 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes