MSH6 Recombinant Rabbit Monoclonal Antibody [JA84-26]

MSH6 Recombinant Rabbit Monoclonal Antibody [JA84-26]

• Description: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair.
• Product Name Alternative: DNA mismatch repair protein Msh6 antibody G/T mismatch binding protein antibody G/T mismatch-binding protein antibody GTBP antibody GTMBP antibody hMSH6 antibody HNPCC 5 antibody HNPCC5 antibody HSAP antibody MSH 6 antibody MSH6 antibody MSH6_HUMAN antibody mutS (E. coli) homolog 6 antibody MutS alpha 160 kDa subunit antibody MutS homolog 6 (E. coli) antibody mutS homolog 6 antibody MutS-alpha 160 kDa subunit antibody p160 antibody Sperm associated protein antibody
• Abbreviation: hMSH6, GTBP, GTMBP, p160, MSH6
• CAS Number: 9000-83-3
• Swiss Prot: P52701 Human, P54276 Mouse
• Cellular Locus: Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: recombinant protein within Human MSH6 aa 1250-1360 / 1360.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IP
• Positive Control: Hela, human tonsil tissue, mouse liver tissue, mouse colon tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:2,000;IHC-P: 1:50-1:200;IP: 1:10-1:50
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: 153 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes